primers for detection of mouse 18S mRNA, FW: CGCCGCTAGAGGTGAAATTC , RV: CCAGTCGGCATCGTTTATGG, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
42
rCYP2R1 qPCR primers
primers for detection of rat CYP2R1 mRNA, FW: AAACTACAACCAATGTGCTCCG , RV: CTTCCCAAGAAGGCCTCCTGT, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
41
mGAPDH qPCR primers
primers for detection of mouse GAPDH mRNA, FW: GGTCATCATCTCCGCCCC, RV: TTCTCGTGGTTCACACCCATC, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20
Biomedical
Biology
Finland
Oulu
40
mATP5B qPCR primers
primers for detection of mouse ATP5B mRNA, FW: GGTTCATCCTGCCAGAGACTA , RV: AATCCCTCATCGAACTGGACG, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
39
mABC1 qPCR primers
primers for detection of mouse ABC1 mRNA, FW: GCTTGTTGGCCTCAGTTAAGG, RV: GTAGCTCAGGCGTACAGAGAT, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
36
mCYCs qPCR primers
primers for detection of mouse Cytochrome C, Somatic mRNA, FW: CCAAATCTCCACGGTCTGTTC , RV: ATCAGGGTATCCTCTCCCCAG, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
35
mCYP24A1 qPCR primers
primers for detection of mouse CYP24A1 mRNA, FW: CTGCCCCATTGACAAAAGGC, RV: CTCACCGTCGGTCATCAGC, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
34
PGC-1BETA qPCR primers
primers for detection of mouse PGC-1beta mRNA, FW: CTCCAGGCAGGTTCAACCC, RV: GGGCCAGAAGTTCCCTTAGG, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
33
LXRA qPCR primers
primers for detection of mouse Liver X Nuclear Receptor alpha mRNA, FW: CTCAATGCCTGATGTTTCTCCT, RV: TCCAACCCTATCCCTAAAGCAA, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
32
ERRA qPCR primers
primers for detection of mouse Estrogen-related receptor alpha mRNA, FW: ATCTGCTGGTGGTTGAACCTG , RV: AGAAGCCTGGGATGCTCTTG, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
31
CYP27B1 qPCR primers
primers for detection of mouse CYP27B1 mRNA, FW: CCAGAACACCCAGAAGTAACT, RV: TCTGTGGGTTCTTGAATACTAGTC, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
30
SIRT2 qPCR primers
primers for detection of mouse sirtuin2 mRNA, FW: CCAGAACACCCAGAAGTAACT, RV: TCTGTGGGTTCTTGAATACTAGTC, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
29
SIRT1 qPCR primers
primers for detection of mouse sirtuin1 mRNA, FW: CCAGAACACCCAGAAGTAACT, RV: TCTGTGGGTTCTTGAATACTAGTC, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
28
mTAT qPCR primers
primers for detection of mouse Tyrosine aminotransferase mRNA, FW: TGCTGGATGTTCGCGTCAATA, RV: CGGCTTCACCTTCATGTTGTC, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
27
HADHB qPCR primers
primers for detection of mouse hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta mRNA, FW: ACTACATCAAAATGGGCTCTCAG, RV: AGCAGAAATGGAATGCGGACC, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
26
HADHA qPCR primers
primers for detection of mouse hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha mRNA, FW: TGCATTTGCCGCAGCTTTAC, RV: GTTGGCCCAGATTTCGTTCA, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
25
mPPARA qPCR primers
primers for detection of mouse Peroxisome Proliferator Activated Receptor Alpha mRNA, FW: AGAGCCCCATCTGTCCTCTC, RV: ACTGGTAGTCTGCAAAACCAAA, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
24
VDRBP qPCR primers
primers for detection of mouse VDR binding protein mRNA, FW: CCTGCTGGCCTTAGCCTTT, RV: TGCTCAAATGTGCTACTGGAAA, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
23
ANGPTL4 qPCR primers
primers for detection of mouse ANGPTL4, FW: AAGATGCACAGCATCACAGG , RV: ATGGATGGGAAATTGGAGC, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
22
ANGPTL3 qPCR primers
primers for detection of mouse ANGPTL3, FW: CCAGAACACCCAGAAGTAACT, RV: TCTGTGGGTTCTTGAATACTAGTC, Concentration used in qPCR is 250 nM, Stock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
21
mRIP140 qPCR primers
primers for detection of mouse RIP140 (Receptor Interacting Protein of 140 kDa)FW1: AGACCAGAACTTTAACCTCTCGGRV1: CGATGGAATCAGACAGCCTCTFW2:TGTCTTAACTTACCTCGAAGGGTRV2:CGTCTGGGTACTGACAGTGGConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
20
mLRP2 qPCR primers
1
-20 C
Biomedical
Biology
Finland
Oulu
19
FGF21 qPCR primers
primers for detection of mouse FGF21FW: GTGTCAAAGCCTCTAGGTTTCTTRV: GGTACACATTGTAACCGTCCTCConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
18
NR1D1 qPCR primers
primers for detection of mouse Nuclear Receptor Subfamily 1 Group D Member 1FW: TCCCCAAGAGAGAGAAGCAARV: CTGAGAGAAGCCCACCAAAGConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
17
CREBH qPCR primers
primers for detection of mouse CREBH (cAMP responsive element binding protein 3 like 3)FW: GTGTCACACCAGGGAGCAAGRV: CAGTGAGGTTGAAGCGGGAGConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
16
Fetuin A qPCR primers
primers for detection of mouse Fetuin AFW: ATCCGCTCCACAAGGTACAGRV: GGTCCAAAGCATGGCAAGTConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
15
CRP qPCR primers
primers for detection of mouse C-Reactive protein mRNA.FW: GGCCAGATGCAAGCATCATCRV: CTGGAGATAGCACAAAGTCCCACConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
14
AKT3 qPCR primers
primers for detection of mouse AKT Serine/Threonine Kinase3FW: TGGGTTCAGAAGAGGGGAGAARV: AGGGGATAAGGTAAGTCCACATCConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
13
AKT2 qPCR primers
primers for detection of mouse AKT Serine/Threonine Kinase2FW: ACGTGGTGAATACATCAAGACCRV: GCTACAGAGAAATTGTTCAGGGGConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
12
AKT1 qPCR primers
primers for detection of mouse AKT Serine/Threonine Kinase1FW: ATGAACGACGTAGCCATTGTGRV: TTGTAGCCAATAAAGGTGCCATConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
11
VDR qPCR primers
primers for detection of mouse vitamin D receptor mRNA.FW: GAATGTGCCTCGGATCTGTGGRV: GGTCATAGCGTTGAAGTGGAAConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical
Biology
Finland
Oulu
10
Mouse Primary Hepatocytes
The mouse primary hepatocytes could be obtained from mice by two-step collagenase liver perfusion. If you are interested in obtaining mouse primary hepatocytes, please contact prof. Jukka Hakkola (jukka.hakkola@oulu.fi).
1
-70 C
Biomedical
Biology
Finland
Oulu
9
Mouse model of type 1 diabetes ( STZ-model)
Type 1 diabetic phenotype was induced by injecting the mice with either vehicle (sod. citrate buffer pH 4.5) or with STZ (60 mg/kg) once daily for 5 days. All mice were kept for a total period of 13 weeks. Additionally, one group of diabetic mice were treated with INSULIN twice a day for 4 weeks before sacrifice. Groups were as follow: 1-vehicle group (non-diabetic controls)= 10 mice 2-STZ group (diabetic mice left untreated)=14 mice 3-STZ + Insulin group (diabetic mice with insulin injections for 4 weeks before sacrificing= 10 mice.
Tissues collected include liver, kidney, heart, muscles, duodenum, ileum, colon, different areas of the brain, brown adipose tissue, testis, and plasma. There is also paraffin blocks from some of these tissues
1
-70 C
Biomedical
Biology
Finland
Oulu
8
Mouse experiment (Knockdown the estrogen-related receptor alpha (ERRa) in the liver)
C57BL/6N mice, aged 8-9 weeks were inject with either SCR-Ad or ERRA-shRNA-Ad (5*10^9 IFU/mouse) via tail vein. The mice were kept for 8 days in total. Before scarifying, the mice were fed or 12h fasted. the treated mice were as follows SCR-Ad (fed or 12h fast) 10 mice/group and ERRA-shRNA-Ad (fed or 12 fasted) 10 mice/group, non-infected controls (fed or 12h fasted) 10 mice/group. the collected tissues include the Liver, Kidney, white adipose tissue, muscles, and Duodenum.
1
-70 C
Biomedical
Biology
Finland
Oulu
7
Mouse experiment (Knockdown the glucocorticoid receptor in the liver)
C57BL/6N mice, aged 8-9 weeks were inject with either SCR-Ad or GR-shRNA-Ad (2,4*10^9 IFU) via tail vein. The mice were kept for 8 days in total. Before sacrifying, the mice were fed or 12h fasted. the treated mice were as follows SCR-Ad (fed or 12h fast) 10 mice/group and GR-shRNA-Ad (fed or 12 fasted) 10 mice/group. the collected tissues include the Liver, Kidney, white adipose tissue, muscles, and Deudenum.
1
-70 C
Biomedical
Biology
Finland
Oulu
6
Mouse experiment (Inhibit the Glucocorticoid receptor in mouse liver by Mifepristone)
The aim was to inhibit the liver GR by GR antagonist mifepristone. C57BL/6N mice, aged 8-9 weeks, were injected with mifepristone (i.p. 100 mg/kg) or vehicle (DMSO/corn oil) two times (the first injection at 9 am and the second injection at 9 pm) and subsequently the mice were either fed or fasted overnight for additional 12h and the tissues were collected. the treated groups were as follow: Vehicle (fed or 12hfast) 8 mice/group and MIF (fed or 12h fast) 8 mice/group. the collected tissues include Liver, Kidney, white adipose tissue, muscles, whole heart, lung, Deudenum, colon, and brown adipose tissues.
1
-70 C
Biomedical
Biology
Finland
Oulu
5
Mouse experiment (Mifepristone plus Dexametahsone)
The aim was to inhibit the GR and abolish the effect of Dexamethasone on the gene of interest in the liver. C57BL/6N mice aged 8 weeks were injected fasted 1 h before an i.p. injection of dexamethasone (3 mg/kg) dissolved in DMSO/corn oil or vehicle only. Simultaneously with the dexamethasone injections, the GR was inhibited with the GR antagonist mifepristone (i.p. 100 mg/kg) dissolved in DMSO/corn oil. The treated groups are as follows Vehicle, DEXA only, MIF only, and DEXA+MIF, 7 mice/group. The collected tissues include the Liver, Kidney, white adipose tissue, and muscles.
1
-70 C
Biomedical
Biology
Finland
Oulu
4
Mouse experiment (Dexamethasone treatment)
C57BL/6N mice aged 8 weeks were fasted 1 h before an i.p. injection of dexamethasone (3 mg/kg) dissolved in DMSO/corn oil or vehicle only. The mice have fasted 6 h and the tissues collected. The treated groups were as follows vehicle and Dexa, 7 mice/group. The collected tissues include the Liver, Kidney, white adipose tissue, and muscles.
primers for detection of mouse Estrogen-related receptor gammaFW1: GAATCTTTTTCCCTGCACTACGARV1: GCTGGAATCAATGTGTCGATCTTFW2: GACCCTACTGTCCCCGACAGTRV1: AACTCTCGGTCAGCCAAGTCAConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
primers for detection of mouse Estrogen-related receptor betaFW: CAGATCGGGAGCTTGTGTTCRV: TGGTCCCCAAGTGTCAGACTConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
primers for detection of mouse Glucocorticoid receptor (NR3C1).FW: AGCTCCCCCTGGTAGAGACRV: GGTGAAGACGCAGAAACCTTGConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
primers for detection of mouse Angioboitin-like protein 8FW: CACTGTACGGAGACTACAAGTGCRV: GTGGCTCTGCTTATCAGCTCGConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20C
Biomedical science
Biology, Biochemistry, Pharmacology, Physiology
Finland
Oulu
45
mPgc1a qPCR primers
primers for detection of mouse PGC-1aFW: TCCTCCTCATAAAGCCAACCRV: GCCTTGGGTACCAGAACACTConcentration used in qPCR is 250 nMStock100 uM, working dilution is 10 uM.
1
-20 C
Biomedical science
Biology, Physiology, Pharmacology, Biochemistry
Finland
Oulu
44
mCyp2r1 qPCR primers
primers for detection of mouse CYP2R1FW: AAACTACAACCAATGTGCTCCG, RV: ATTCCCAAGAAGGTCTCCTGT, Concentration used in qPCR is 250 nM Stock 100 uM, working dilution is 10 uM.
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